May, 22, 2016, Creative Proteomics, a leading supplier in drug development services, carried out its new services for determination of amino acid, which is named as the first brick of proteins in human beings bodies.
In Creative Proteomics, scientists can conduct three methods for analyzing amino acid, they are HPLC, automatic amino acid analyzer and LC-MS/MS. Among these methods, HPLC is the most popular method for determining amino acid components.
Detection methods
The method--UV detection needs the use of absorption of the carboxyl group, which is in the 200 to 210 nm range, fo the determination of amino acid. However, some amino acids with benzene rings are difficult to be analyzed because of the sufficient sensitivity and selectivity.
This post is used to introduce the pre-column derivatization method and post-column derivation method.
Pre-column derivatization method
With this method, amino acid should be derivertized before injection, and then detecting and separating the reaction products. The advantages of this method are listed below:
1. Specifying a small reaction system in this method can minimize the reagent consumption rates.
2. This method is also can increase the sensitivity with more expensive reagents and provide lower background levels, which is lower than post-column derivatization method.
3. There is no problem for detecting the reagents, which have not reacted, once it is separated in the column.
However, the disadvantages are also lying in that the reaction efficiency can be easily affected by the sample matrix mixed with derivatizing reagents.
Post-column derivatization method
The post-column derivatization method contains several steps, including separation of the amino acid in the column, the delivery and mix of the derivatizing reagent before sending the products to the detector. Some advantages of this method are listed as below:
1. This method can offer excellent quantitative performance and reproducibility automatly.
2. Reaction efficiency will be less prone to sample matrix effects because the sample components have been separated before the reaction. So this allows this method can be widely applied in a wide range of samples.
However, its disadvantages are the difficulty in increasing sensitivity and high consumption of reaction reagent, which is kept constantly flowing.
These are some basic information about pre-column derivatization method and post-column derivatization method, which is widely used in the determination of amino acid.
In Creative Proteomics, scientists can conduct three methods for analyzing amino acid, they are HPLC, automatic amino acid analyzer and LC-MS/MS. Among these methods, HPLC is the most popular method for determining amino acid components.
Detection methods
The method--UV detection needs the use of absorption of the carboxyl group, which is in the 200 to 210 nm range, fo the determination of amino acid. However, some amino acids with benzene rings are difficult to be analyzed because of the sufficient sensitivity and selectivity.
This post is used to introduce the pre-column derivatization method and post-column derivation method.
Pre-column derivatization method
With this method, amino acid should be derivertized before injection, and then detecting and separating the reaction products. The advantages of this method are listed below:
1. Specifying a small reaction system in this method can minimize the reagent consumption rates.
2. This method is also can increase the sensitivity with more expensive reagents and provide lower background levels, which is lower than post-column derivatization method.
3. There is no problem for detecting the reagents, which have not reacted, once it is separated in the column.
However, the disadvantages are also lying in that the reaction efficiency can be easily affected by the sample matrix mixed with derivatizing reagents.
Post-column derivatization method
The post-column derivatization method contains several steps, including separation of the amino acid in the column, the delivery and mix of the derivatizing reagent before sending the products to the detector. Some advantages of this method are listed as below:
1. This method can offer excellent quantitative performance and reproducibility automatly.
2. Reaction efficiency will be less prone to sample matrix effects because the sample components have been separated before the reaction. So this allows this method can be widely applied in a wide range of samples.
However, its disadvantages are the difficulty in increasing sensitivity and high consumption of reaction reagent, which is kept constantly flowing.
These are some basic information about pre-column derivatization method and post-column derivatization method, which is widely used in the determination of amino acid.
