Dolomite, a world leader in microfluidics innovation, has launched the Single Cell RNA-Seq Chip for simple, reliable creation of single cell libraries. This new chip allows researchers to use droplet-based microfluidic methods to help explore the behaviour of thousands of individual cells, providing a rapid and easy way to screen for new or cryptic cell types.
The Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop-seq chip described by Macosko et al1 – is manufactured from glass, offering excellent optical transparency and ensuring high precision. Combined with a very gentle sample loop method – to minimise bead shearing and contamination due to premature cell lysis – it enables rapid, straightforward preparation of single cell cDNA libraries. The chip offers robust and reproducible processing of 1 ml samples in approximately 12 hours, allowing cells to be individually encapsulated with uniquely barcoded RNA capture beads for high throughput sequencing.
To find out more, please visit http://www.dolomite-microfluidics.com/webshop/microfluidic-chips-droplet-chips-single-cell-rna-seq-chip-c-17_19_126/single-cell-rnaseq-chip-1-100-microetch-depth-fluorophilic-p-419
1. Macosko, Evan Z. et al (2015). Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell, 161(5): 1202-1214.
About Dolomite
Established in 2005, Dolomite Microfluidics has grown to be the world leader in the design and manufacture of high quality innovative microfluidic products.
The company offers a broad range of modular microfluidic systems, modules and components – including pumps, chips, connectors, temperature controllers, sensors, accessories and custom-made components – as well as software for analysis or automation.
Modularity, ease of use, innovation and scalability are common to all Dolomite products, which are used across a broad range of applications in biology, drug discovery, chemistry, food, cosmetics and academia.
Dolomite is a part of the Blacktrace group of companies, a world leader in Productizing Science®, and has offices in the USA, Japan, India and Brazil, as well as a worldwide network of distributors.
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