ROCKFORD, Ill. (April 14, 2009) — Thermo Fisher Scientific, the world leader in serving science, introduces two Thermo Scientific In-Cell ELISA Detection Kits to provide a simple and convenient method for quantifying intracellular proteins in whole cells.
The Thermo Scientific In-Cell ELISA Near Infrared Detection Kit enables the simultaneous detection of two different target proteins within the same well (e.g., a post-translational-modified protein and its unmodified form), eliminating the variability caused by differences in cell plating. The kit uses primary antibodies to the target protein(s) and corresponding species-specific Thermo Scientific DyLight-conjugated Secondary Antibodies to monitor target protein(s) levels. The kit is compatible with the popular Odyssey Infrared Imaging System.
The Thermo Scientific In-Cell ELISA Colorimetric Detection Kit uses target-specific primary antibodies and a horseradish peroxidase (HRP)-conjugated detection reagent to detect proteins of interest. To control for differences arising from cell plating, a whole-cell stain is included for normalization.
The kits enable accurate quantitation using either a standard ELISA reader (colorimetric detection) or an infrared imaging system (near infrared detection) and offer higher throughput over traditional Western blotting methods. The assays are performed in 96- or 384-well microplates, making the assays amenable to automation which is ideal for siRNA studies and drug screens.
Both the Near Infrared Kit and the Colorimetric Kit supply sufficient materials for 4 x 96 wells. The kits can be stored at 4°C for up to one year. For more information, please visit www.thermo.com/pierce or call +1-815-968-0747.
Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
